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Topflash/fopflash reporter

WebLentiviral-TOP-dGFP-reporter FOP-GFP.mC M50 Super 8x TOPFlash Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn about the latest plasmid technologies and research tools. Subscribe. Contact Addgene. Have questions about your order, deposit, or a plasmid? ... WebThe first transcription-based luciferase reporter of Wnt/β-catenin signaling, TOPFlash, was designed by Korinek et al. . The TOPFlash reporter contains three TCF response elements (CCTTTGATC) upstream of a basal c-fos promoter while the control reporter, FOPFlash, contains three mutant TCF response elements (CCTTTG GC C). TOP-Flash was later ...

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WebTOPflash (TCF-Reporterplasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase minimal promoter and Luciferase open reading frame.; find Sigma-Aldrich-21170 MSDS, related peer-reviewed papers, … Web用western和免疫荧光验证beta－catenin和下游靶基因的变化只是基础，只是说明你转染了这个抑癌基因的确是改变了catenin通路，但是你想要证明该抑癌基因是通过catenin通路来 … inattentively meaning https://colonialfunding.net

Inhibition of β-catenin/B cell lymphoma 9 protein−protein …

Web4. apr 2024 · Biological and molecular characteristics of GC cell lines and PDOs upon PMCA4 inhibition were investigated using RT-qPCR, western blot, and TOPflash/FOPflash reporter assay. WebAddgene inc topflash fopflash luciferase reporter plasmids Topflash Fopflash Luciferase Reporter Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Web14. máj 2024 · TOPFlash/FOPFlash Luciferase Reporter Assays. Wnt-specific TOPFlash/FOPFlash luciferase reporter assays were used to evaluate the effects of these compounds on β-catenin–dependent transcription. in air wifi

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Topflash/fopflash reporter

TOPFlash(报告基因质粒)(D2501-1μg)

WebTop和Fop序列基本上一致的，Top的荧光素酶启动子上有几个Lef/TCF的结合位点，而在FOp是 突变 的，因此Top/Fop可以检测Wnt的活性改变。 1.应该是要用Top/FOP值来表示 … WebI did lipofectamine transfection in 12-well plates with 0.5M of cells and 0.8ug of TOPFLASH plasmid in each well. After 24h, I changed to media to serum-free media supplemented …

Topflash/fopflash reporter

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WebFrom what I can understand, TOPflash is a luciferase reporter plasmid that contains two sets of 3 copies of the wild-type TCF binding regions. If the canonical Wnt signaling is … Web26. dec 2024 · topflash is a tcf reporter plasmid containing two sets of three copies of wild-type tcf binding sites driven by the thymidine kinase minimal prompter and upstream of a …

WebJournal: PLoS ONE Article Title: The natural compound Jatrophone interferes with Wnt/β-catenin signaling and inhibits proliferation and EMT in human triple-negative breast cancer doi: 10.1371/journal.pone.0189864 Article Snippet: The activity of β-catenin dependent Wnt signaling was measured by the TOPFLASH reporter using the Dual Luciferase Reporter … WebFOPFlash(报告基因质粒)是TOPFlash(D2501)的阴性对照报告基因质粒。 ... Reporter Vector primer 4 (RVprimer4) binding region: 3537-3556: ColE1-derived plasmid replication origin: …

WebTo measure Wnt signaling, one can use a variety of Wnt reporters. The TOP-flash assay ( Molenaar 1996) is widely used and variants of TOP-Flash (SuperTop) are available from … Web浩格生物科技有限公司是专业的35S-Mt-Hc-mcherry(Kan)HH-ZW-060厂家,为您提供价格优惠最佳解决方案,我们有35S-Mt-Hc-mcherry(Kan)HH-ZW-060各种型号、资料、使用说明PDF文档,采购35S-Mt-Hc-mcherry(Kan)HH-ZW-060尽在浩格生物科技有限公司

WebThis is a luciferase reporter of beta-catenin-mediated transcriptional activation. In HEK cells, maximal activation of this reporter is ~100-fold (activation by Wnt) up to ~1,000-fold …

Web15. máj 2024 · While all three compounds had comparable inhibitory effect on the TopFlash reporter above 2.5 µM, compounds C11 and C16 also negatively affected the FopFlash reporter, thus indicating... inattentive type of adhdWebSKL2001 is a specific small molecule activator (agonist) of the Wnt/β-catenin pathway, stabilizes intracellular β-catenin via disruption of the Axin/β-catenin interaction. SKL2001 strongly activates β-catenin responsive transcription, upregulates TOPflash reporter activity without effect on the activity of FOPFlash with mutated β-catenin/Tcf-binding elements, … inattentiveness antonymWeb1169.00元. TOPFlash(报告基因质粒)是用于检测Wnt信号通路中β-catenin介导的TCF/LEF转录活性水平的报告基因质粒。. TOPFlash是以碧云天的pGL6-TA为模板，在其多克隆位点插 … in air what is the solventWebDescription. FOPflash: Transfection grade T cell factor (TCF) reporter plasmid containing two full and one incomplete copy of the TCF binding site (mutated) followed by three … inattentively definitionWebUpstate Biotechnology Inc luciferase reporter plasmids topflash. Luciferase Reporter Plasmids Topflash, supplied by Upstate Biotechnology Inc, used in various techniques. … inattentiveness in spanishTOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase minimal promoter and Luciferase open reading frame. inattentiveness during meetingsWebUpstate Biotechnology Inc topflash fopflash luciferase reporter constructs Topflash Fopflash Luciferase Reporter Constructs, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more in air mouse